K+ influx components in ascites cells: The effects of agents interacting with the (Na++K+)-pump |
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Authors: | Tilly Bakker-Grunwald J Scott Andrew Margaret C Neville |
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Institution: | (1) Department of Physiology, University of Colorado Medical Center, 80262 Denver, Colorado |
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Abstract: | Summary Several agents known to interact with the (Na++K+)-pump were tested for their effects on the components of steady-state K+ flux in ascites cells.86Rb+ was used as a tracer for K+, and influx was differentiated into a ouabain-inhibitable pump component, a Cl–-dependent and furosemide-sensitive exchange component, and a residual leak flux. All agents tested (ouabain, quercetin, oligomycin, phosphate) affected both the pump flux and the Cl–-linked flux. These findings suggest a linkage between the activity of the Na/K ATPase and the Cl–-dependent K+ exchange flux. In the discussion we point out that the mechanism of this linkage could be direct; e.g., Cl–-dependent exchange may represent a mode of operation of the Na/K ATPase. However, data from this and other systems tend to suggest an indirect linkage between the Na+ pump and a KCl symporter, perhaps via a change in the level of intracellular ATP. |
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