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Novel synthesized colourless ligands in affinity chromatography for purification of alkaline phosphatase
Authors:Gao Kunyu  Yang Li  Cheng Lubai
Affiliation:(1) Department of Fine Chemicals, College of Chemical Engineering, Dalian University of Technology, 116012 Dalian, People's Republic of China
Abstract:Summary A 350-and a 460-fold one step purification of alkaline phosphatase from a crude calf intestine extract has been achived using two new synthesized colourless biomimetic compounds coupled to Agarose CL-4B.
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