Bacteriocin Release Protein Triggers Dimerization of Outer Membrane Phospholipase A In Vivo |
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| Authors: | Niek Dekker Jan Tommassen Hubertus M. Verheij |
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| Affiliation: | Department of Enzymology and Protein Engineering, Center for Biomembranes and Lipid Enzymology, Utrecht University, 3584 CH Utrecht, The Netherlands. n.dekker@chem.uu.nl |
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| Abstract: | Bacteriocin release protein is known to activate outer membrane phospholipase A (OMPLA), which results in the release of colicin from Escherichia coli. In vivo chemical cross-linking experiments revealed that the activation coincides with dimerization of OMPLA. Permeabilization of the cell envelope and dimerization were characterized by a lag time of 2 h. |
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