Salt-stable association of simian virus 40 capsid with simian virus 40 DNA |
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Authors: | V Blasquez M Bina |
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Affiliation: | Chemistry Department, Purdue University, West Lafayette, IN 47907, USA |
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Abstract: | In 8 M CsCl, a fraction of the wild-type previrions and tsB228 nucleoprotein complexes lose their core histones but retain their capsid. These histone-depleted complexes appear in the electron microscope as a protein shell attached to supercoiled DNA. Consistent with this result, we find that in 1 M NaCl, the wild-type previrions dissociate into two populations of nucleoprotein complexes. One population sediments between 50 and 140 S and morphologically resembles the shell-DNA complexes isolated in CsCl gradients. The other population is comprised primarily of nucleoproteins which sediment at 40 S. |
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