On-line biological sample cleanup for electrospray mass spectrometry using sol-gel columns |
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Authors: | Johannesson Nina Pearce Evan Dulay Maria Zare Richard N Bergquist Jonas Markides Karin E |
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Institution: | Uppsala University, Department of Physical and Analytical Chemistry, Uppsala, Sweden. |
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Abstract: | Using a slight overpressure, a urine sample is loaded onto a monolithic photopolymerized sol-gel column that has been derivatized with hydrophobic carbon chains and then the complex urine matrix is washed with aqueous solution. A buffer containing organic solvent is used to elute the adsorbed peptides by an applied voltage and the sample is then introduced into a mass spectrometer by sheath flow electrospray. The importance of desalting this type of sample is demonstrated by an experiment that shows that the signal intensity of a test solution with neurotensin, sprayed directly into the mass spectrometer, decreased from 4.5x10(4) cps to no detectible signal when just 10% urine is added to the sample solution. We suggest that this procedure may find general application for desalting biological samples prior to mass spectrometric analysis. |
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