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Biopolymer of alginate nature with a predominance of L-guluronic acid
Authors:Ya. O. Loginov  G. G. Khudaigulov  S. P. Chetverikov  A. I. Melent’ev  O. N. Loginov
Affiliation:(1) Key Laboratory of Molecular MicroBiology and Technology, Ministry of Education, Department of MicroBiology, College of Life Sciences, Nankai University, Tianjin, 300071, China;(2) The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin, 300071, China;
Abstract:Highly viscous polysaccharide (250–350 kDa) of an alginate nature with a predominance of α-L-guluronic acid (M/G = 0.22) was obtained from Azotobacter vinelandi. The yield of polysaccharide was 20.5 ± 0.5 g/l when cultured in a medium containing molasses at a viscosity of the cultural liquid of over 30000 cSt. The biopolymer is stable at pH 4.0–9.0 in a wide temperature range and well soluble in highly mineralized water; it retains a high viscosity level and can be used in the petroleum industry for enhanced oil recovery.
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