Optimization of the <Emphasis Type="Italic">Lactococcus lactis</Emphasis> nisin-controlled gene expression system NICE for industrial applications |
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Authors: | Email author" target="_blank">Igor?MierauEmail author Kees?Olieman James?Mond Eddy?J?Smid |
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Institution: | (1) NIZO food research, P.O. Box 20, 6710 BA EDE, The Netherlands;(2) Biosynexus Inc., 9119 Gaither Road, 20877 Gaithersburg, MD, USA |
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Abstract: | Background The nisin-controlled gene expression system NICE of Lactococcus lactis is one of the most widely used expression systems in Gram-positive bacteria. Despite its widespread use, no optimization
of the culture conditions and nisin induction has been carried out to obtain maximum yields. As a model system induced production
of lysostaphin, an antibacterial protein (mainly against Staphylococcus aureus) produced by S. simulans biovar. Staphylolyticus, was used. Three main areas need optimization for maximum yields: cell density, nisin-controlled
induction and protein production, and parameters specific for the target-protein. |
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