Affiliation: | (1) NIZO food research, P.O. Box 20, 6710 BA EDE, The Netherlands;(2) Biosynexus Inc., 9119 Gaither Road, 20877 Gaithersburg, MD, USA |
Abstract: | Background The nisin-controlled gene expression system NICE of Lactococcus lactis is one of the most widely used expression systems in Gram-positive bacteria. Despite its widespread use, no optimization of the culture conditions and nisin induction has been carried out to obtain maximum yields. As a model system induced production of lysostaphin, an antibacterial protein (mainly against Staphylococcus aureus) produced by S. simulans biovar. Staphylolyticus, was used. Three main areas need optimization for maximum yields: cell density, nisin-controlled induction and protein production, and parameters specific for the target-protein. |