Creation of a heterologous gene expression system on the basis of Aspergillus awamori recombinant strain |
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| Authors: | A. M. Rozhkova A. S. Sereda N. V. Tsurikova A. K. Nurtaeva M. V. Semenova L. V. Rimareva E. A. Rubtsova I. N. Zorov O. A. Sinitsyna A. P. Sinitsyn |
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| Affiliation: | 1.Bach Institute of Biochemistry,Russian Academy of Sciences,Moscow,Russia;2.Institute of Food Biotechnology,Russian Academy of Agricultural Sciences,Moscow,Russia;3.Kazakhstan Institute of Management and Economics,Almaty,Kazakhstan;4.Department of Chemistry,Moscow State University,Moscow,Russia |
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| Abstract: | A heterologous gene expression system was created in a domestic Aspergillus awamori Co-6804 strain, which is a producer of glucoamylase. Vector pGa was prepared using promoter and terminator areas of the glucoamylase gene, and Aspergillus niger phytase, Trichoderma reesei endoglucanase, and Penicillium canescens xylanase genes were then cloned into pGa vector. Separation of enzyme samples using FPLC showed the amount of the recombinant proteins to be within the 0.6–14% range of total protein. |
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