Production of enzyme preparations on the basis of Penicillum canescens recombinant strains with a high ability for the hydrolysis of plant materials |
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Authors: | P. V. Volkov A. M. Rozhkova A. G. Pravilnikov R. M. Andrianov G. S. Dotsenko A. O. Bekkarevich A. V. Koshelev O. N. Okunev I. N. Zorov A. P. Sinitsin |
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Affiliation: | 1. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia 2. Faculty of Chemistry, Moscow State University, Moscow, 119999, Russia 3. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Moskovskaya oblast, 142290, Russia
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Abstract: | An enzyme preparation has been produced on the basis of Penicillium canescens strains with the activity of cellibiohydrolase I, II; endo-1,4-β-gluconase of Penicillium verruculosum; and β-glucosidase of Aspergillus niger. It was shown that for the most effective hydrolysis of aspen wood pulp the optimal ratio of cellobiohydrolase and endo-1,4-β-gluconase in enzyme preparations was 8: 2 (by protein). It was also established that the homologous xylanase secreted by the Penicillium canescens fungus is a required component for the enzyme complex for hydrolysis of the hemicellulose matrix of aspen wood. |
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