Cyclic digene system as a control element of a bacterial biosensor |
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Authors: | E E Stupak I V Stupak |
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Institution: | 1. Institute of Biology, Ufa Scientific Center, Ufa, 450054, Russia
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Abstract: | The Escherichia coli JC158(pCIA12/pGFK5) strain carrying a cyclic digene system with a negative feedback on the pCIA12 plasmid reacting to the
DNA damage by changing the synthesis level of reporter genes—GFP and β-galactosidase—was tested. The acquired phenotype was
inherited by the next generations after the removal of the genotoxic action when the concentration of the DNA-damaging compounds
was above the threshold level. A potential has been shown for the application of bacterial biosensors to monitor the presence
of genotoxicants in the environment and to test the consequences of short-term exposures to toxic compounds. |
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Keywords: | |
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