Antioxidant activity of oxygen-containing aromatic compounds |
| |
Authors: | M V Potapovich V P Kurchenko D I Metelitza O I Shadyro |
| |
Institution: | 1.Research Institute of Physicochemical Problems,Belarusian State University,Minsk,Belarus;2.Biological Faculty,Belarusian State University,Minsk,Belarus |
| |
Abstract: | Inhibition efficiency (antioxidant activity) of 26 oxygen-containing aromatic compounds was studied in methemalbumin-H2O2-o-phenylenediamine (PDA) or tetramethylbenzidine (TMB) pseudoperoxidase system at 20°C in buffered physiological solution (pH
7.4) containing 6% DMF and 0.25% DMSO. The inhibitor’s efficiency was quantitatively characterized by the inhibition constants
(K
i, μM) or the inhibition degree (%). K
i values varied in the range of 4 to 500 μM and were influenced by a substrate, the structure of an inhibitor, hydroxyl groups,
electron-donating substituents in aromatic ring, and steric hindrances. The type of inhibition at cooxidation of eight pairs
was noncompetitive, and that of five pairs was mixed and determined by the substrate nature and the inhibitor structure. Lignin
phenolic compounds of guaiacyl and syringal series exhibited high antioxidant activity (K
i in the range of 10–300 μM), and their efficiency decreased in the following order: caffeic acid > synapaldehyde > syringic
acid > coniferyl aldehyde > para-hydroxycoumaric acid. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|