Desulfosoma profundi sp. nov., a thermophilic sulfate-reducing bacterium isolated from a deep terrestrial geothermal spring in France |
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Authors: | Patrick Grégoire Marie-Laure Fardeau Sophie Guasco Joël Lagière Jean Cambar Valérie Michotey Patricia Bonin Bernard Ollivier |
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Institution: | 1. Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL Case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France 2. Institut du Thermalisme, Université Victor Segalen Bordeaux 2, 8 rue Sainte Ursule, 40100, Dax, France 3. Laboratoire de Microbiologie, de Géochimie et d’Ecologie Marine, Centre d’Océanologie de Marseille, Université de la Méditerranée, Aix Marseille II, CNRS-UMR 6117 Campus de Luminy, Case 901, 13009, Marseille Cedex 9, France
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Abstract: | A novel strictly anaerobic bacterium designated SPDX02-08T was isolated from a deep terrestrial geothermal spring located in southwest France. Cells (1–2 × 2–6 μm) were non-motile,
non sporulating and stained Gram negative. Strain SPDX02-08T grew at a temperature between 40 and 60°C (optimum 55°C), pH between 6.3 and 7.3 (optimum 7.2) and a NaCl concentration between
0 and 5 g/l (optimum 2 g/l). Sulfate, thiosulfate and sulfite were used as terminal electron acceptors, but not elemental
sulfur, nitrate, nitrite, Fe (III) or fumarate. In the presence of sulfate, strain SPDX02-08T completely oxidized pyruvate, propionate, butyrate, isobutyrate, valerate, isovalerate and hexadecanoate. Stoichiometric
measurements revealed a complete oxidation of part of lactate (0.125 mol of acetate produced per mole lactate oxidized). Strain
SPDX02-08T required yeast extract to oxidize formate and H2 but did not grow autotrophically on H2. Among the substrates tested, only pyruvate was fermented. The G+C content of the genomic DNA was 57.6 mol%. Major cellular
fatty acids of strain SPDX02-08T were iso-C15:0, C15:0, and C16:0. Phylogenetic analysis of the 16S small-subunit (SSU) ribosomal RNA gene sequence indicated that strain SPDX02-08T belongs to the genus Desulfosoma, family Syntrophobacteraceae, having Desulfosoma caldarium as its closest phylogenetic relative (97.6% similarity). The mean DNA/DNA reassociation value between strain SPDX02-08T and Desulfosoma caldarium was 16.9 ± 2.7%. Based on the polyphasic differences, strain SPDX02-08T is proposed to be assigned as a new species of the genus Desulfosoma, Desulfosoma profundi sp. nov. (DSM 22937T = JCM 16410T). GenBank accession number for the 16S rRNA gene sequence of strain SPDX02-08T is HM056226. |
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