Evidence for in vitro induced mutation which improves somatic embryogenesis in Asparagus officinalis L. |
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Authors: | B. Delbreil M. Jullien |
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Affiliation: | (1) Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint Cyr, F-78026 Versailles cedex, France;(2) Laboratoire in vitro, Jacques Marionnet GFA, F-41230 Soings en Sologne, France;(3) Present address: Universite des Sciences et Technologies de Lille, Laboratoire de Physiologie Cellulaire et Morphogenèse Végétales, Bât. SN2, F-59655 Villeneuve d'ASCQ Cedex, France |
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Abstract: | Summary Somatic embryogenesis from different genotypes of Asparagus officinalis L. could be obtained by in vitro culture of shoot apices. Apices were first cultured on an auxin-rich inducing medium and then transferred onto a hormone-free development medium. All genotypes tested in this way produced a few somatic embryos. In some experiments, during the development phase, a new kind of friable highly embryogenic tissue appeared in a random manner. These tissues could be continuously subcultured on a hormone-free medium and were named embryogenic lines. Five of these embryogenic lines regenerated plants from somatic embryos. These regenerated plants exhibited an increased embryogenic response compared to the parent plants; e.g. apex culture produced somatic embryos without any auxin treatments. For one of the embryogenic lines, a genetic analysis showed that the improved embryogenic response of regenerated plants was controlled by a mendelian dominant monogenic mutation.Abbreviations LSEA low somatic embryogenesis ability - HSEA high somatic embryogenesis ability - NAA 1-naphthaleneacetic acid |
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Keywords: | Asparagus officinalis L Habituation Mutation Somatic embryogenesis |
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