A detailed linkage map of lettuce based on SSAP, AFLP and NBS markers |
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Authors: | Naeem H Syed Anker P Sørensen Rudie Antonise Clemens van de Wiel C Gerard van der Linden Wendy van ‘t Westende Danny A P Hooftman Hans C M den Nijs Andrew J Flavell |
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Institution: | (1) Plant Research Unit, University of Dundee at SCRI, Invergowrie, Dundee, DD2 5DA, UK;(2) Keygene N.V. Applied Research, 216, 6700 Wageningen, AE, The Netherlands;(3) Plant Research International B.V., Wageningen University and Research Centre, 16, 6700 Wageningen, AA, The Netherlands;(4) Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Kruislaan 318, 1098 Amsterdam, SM, The Netherlands |
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Abstract: | Molecular markers based upon a novel lettuce LTR retrotransposon and the nucleotide binding site-leucine-rich repeat (NBS-LRR)
family of disease resistance-associated genes have been combined with AFLP markers to generate a 458 locus genetic linkage
map for lettuce. A total of 187 retrotransposon-specific SSAP markers, 29 NBS-LRR markers and 242 AFLP markers were mapped
in an F2 population, derived from an interspecific cross between a Lactuca sativa cultivar commonly used in Europe and a wild Lactuca serriola isolate from Northern Europe. The cross has been designed to aid efforts to assess gene flow from cultivated lettuce into
the wild in the perspective of genetic modification biosafety. The markers were mapped in nine major and one minor linkage
groups spanning 1,266.1 cM, with an average distance of 2.8 cM between adjacent mapped markers. The markers are well distributed
throughout the lettuce genome, with limited clustering of different marker types. Seventy-seven of the AFLP markers have been
mapped previously and cross-comparison shows that the map from this study corresponds well with the previous linkage map. |
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