Oscillations of Ca++ concentration during the cell differentiation of Dictyostelium discoideum: their relation to oscillations in cyclic AMP and other components |
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| Authors: | Bumann J Malchow D Wurster B |
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| Institution: | 1. Fakultät für Biologie, Universität Konstanz, 7750 Konstanz, Federal Republic of Germany;1. Centre INRS-Institut Armand-Frappier, INRS, Laval, Canada H7V 1B7;2. Department of Anatomy and Cell Biology, McGill University, Montreal, Canada H3A 2B2;3. Département d’Ophtalmologie et Institut de Génie Biomédical, Université de Montréal, Montréal, Canada H3T 1J4;4. Centre de Recherche de l’Hôpital Maisonneuve-Rosemont, Montréal, Canada H1T 2M4;5. Biomedical Center (BMC), Institute for Metabolic Biochemistry, Ludwig-Maximilians University Munich, Munich, Germany;6. DZNE – German Center for Neurodegenerative Diseases, Munich, Germany;1. Ghent University Hospital, Dpt. Haematology, C. Heymanslaan 10, 9000 Ghent, Belgium;2. Ghent University Hospital, Laboratory of Molecular Diagnostics, C. Heymanslaan 10, 9000 Ghent, Belgium;3. Ghent University Hospital, Dpt. Laboratory Medicine, C. Heymanslaan 10, 9000 Ghent, Belgium;4. AZ Delta, Dpt. Haematology, Wilgenstraat 2, 8800 Roeselare, Belgium;5. AZ Sint-Jan, Dpt. Haematology, Ruddershove 10, 8000 Brugge, Belgium;6. ZNA Stuivenberg, Dpt. Haematology, Lange Beeldekensstraat 267, 2060 Antwerpen, Belgium;7. University Hospital Saint-Luc, Dpt. Haematology, Avenue Hippocrate 10, 1200, Brussels, Belgium;8. Ghent University Hospital, Dpt. Haematology, C. Heymanslaan 10, 9000, Ghent, Belgium;1. Pauley Heart Center, Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University, Richmond, VA, 23298, United States;2. Department of Biochemistry & Molecular Biology, Virginia Commonwealth University, Richmond, VA, 23298, United States;1. Department of Translational and Precision Medicine, Sapienza University of Rome, Rome, Italy;2. Department of Pediatric Hematology and Oncology, St. Anna Children''s Hospital, Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Austria;3. Center of Biostatistics for Clinical Epidemiology, Department of Health Science, University of Milano-Bicocca, Milano, Italy;4. Department of Pediatrics, Christian-Albrechts-University Kiel and University Medical Center Schleswig-Holstein, Kiel, Germany;5. Department of Pediatric Oncology, University Children''s Hospital, Zürich, Switzerland;6. Pediatric Hematology-Oncology Unit, Department of Pediatrics, University of Milano-Bicocca, MBBM Foundation/ASST Monza, Monza, Italy;7. Department of Children and Adolescent Medicine, Children''s Hospital, Goethe University, Frankfurt, Germany;8. Department of Pediatric and Adolescent Medicine, University Children''s Hopsital Augsburg, Swabian Children''s Cancer Center, Augsburg, Germany;9. Department of Pediatric Hemato-Oncology, A.O.R.N. Santobono-Pausilipon, Naples, Italy;10. Department of Woman and Child Health, Laboratory of Haematology-Oncology, University of Padova, Padova, Italy;11. Department of Pediatric Hematology and Oncology, Children''s Hospital, Eberhard Karls University, Tübingen, Germany;12. Department of Pediatric Hematology and Oncology, Children''s Hospital, Albert Ludwigs University, Freiburg, Germany;13. Pediatric Onco-Hematology, Regina Margherita Children''s Hospital, AOU Città della Salute e della Scienza, Turin, Italy;14. Department of Pediatric Hematology-Oncology, IRCCS “Bambino Gesù” Children''s Hospital, Rome, Italy;15. Department of Pediatrics, Sapienza, University of Rome, Rome, Italy;p. Department of Pediatric Hematology and Oncology, Children''s Hospital, Medical School Hannover, Germany;q. Department of Pediatric and Adolescent Medicine, Children''s Hospital, University of Lübeck, Germany;r. Department of Pediatrics, Pediatric Hemato-Oncology Unit, Children''s Hospital “Carl Gustav Carus”, Dresden, Germany;s. Italian Institute for Genomic Medicine, Turin, Italy |
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| Abstract: | Periodic cyclic-AMP pulses control the cell aggregation and differentiation of Dictyostelium discoideum. Another component required for the aggregation and differentiation of these cells appears to be extracellular Ca+ +. Oscillations in extracellular Ca+ + concentration were investigated in suspensions of differentiating cells. We observed spike-shaped and sinusoidal Ca+ + oscillations. In the course of differentiation, spike-shaped Ca+ + oscillations preceded sinusoidal oscillations, and no phase change occurred at the transition from spike-shaped to sinusoidal Ca+ + oscillations. Spike-shaped and sinusoidal Ca+ + oscillations were related to oscillations in (1) the cyclic-AMP and cyclic-GMP content of cells, (2) the light-scattering properties of cells, and (3) the extracellular pH. Spikeshaped Ca+ + oscillations were observed together with cyclic-AMP oscillations. The minima of the extracellular Ca+ + concentration trailed the maxima of the cyclic-AMP concentration by about 30 s. Sinusoidal Ca+ + oscillations were not accompanied by measurable cyclic-AMP oscillations. The amplitudes of the sinusoidal Ca+ + oscillations were smaller than those of the spike-shaped Ca+ + oscillations. A Ca+ + oscillation of small amplitude (instead of a spike-shaped oscillation) was observed when one cyclic-AMP spike was skipped. Our results provide evidence for the existence of a sinusoidal cyclic-AMP-independent Ca+ + oscillation of small amplitude, and they also suggest that spike-shaped Ca+ + oscillations may be superimposed on such small-amplitude oscillations. When D. discoideum cells produce cyclic-AMP spikes, the uptake of additional Ca+ + is induced, resulting in Ca+ + oscillations of a large amplitude. |
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