N-terminal extension of Saccharomyces cerevisiae translation termination factor eRF3 influences the suppression efficiency of sup35 mutations |
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Authors: | Volkov Kirill Osipov Kirill Valouev Igor Inge-Vechtomov Sergey Mironova Ludmila |
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Institution: | Department of Genetics, St Petersburg State University, St Petersburg, Russia. |
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Abstract: | The eukaryotic translation termination factor eRF3 stimulates release of nascent polypeptides from the ribosome in a GTP-dependent manner. In most eukaryotes studied, eRF3 consists of an essential, conserved C-terminal domain and a nonessential, nonconserved N-terminal extension. However, in some species, this extension is required for efficient termination. Our data show that the N-terminal extension of Saccharomyces cerevisiae eRF3 also participates in regulation of termination efficiency, but acts as a negative factor, increasing nonsense suppression efficiency in sup35 mutants containing amino acid substitutions in the C-terminal domain of the protein. |
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Keywords: | nonsense suppression translation termination eRF3 yeast |
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