Comparative studies of host-cell reactivation, colony forming ability and excision repair after UV irradiation of xeroderma pigmentosum, normal human and some other mammalian cells |
| |
Authors: | H Takebe S Nii M I Ishii H Utsumi |
| |
Institution: | 1. Department of Fundamental Radiology, Faculty of Medicine, Osaka University, Kita-ku, Osaka 530, Japan;1. Department of Pathology, Research Institute for Microbial Diseases, Osaka University, Yamadakami, Suita 565, Japan;7. Department of Experimental Radiology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606, Japan |
| |
Abstract: | Host-cell reactivation, that is, the degree of survival of Herpes simplex virus after UV irradiation, was high in African green monkey BSC-1 cells, intermediate in normal human fibroblasts and human FL cells, and low in both xeroderma pigmentosum (XP) cells and mouse L cells. However, colony-forming ability after UV was high for FL, normal human fibroblasts and L cells, slightly low for BSC-1 cells and extremely low for XP cells. During the 24-h post-UV incubation period, up to about 50% of the thymine-containing dimers in the acid-insoluble DNA fraction disappeared at an almost equal rate for BSC-1, FL and normal human cells but remained unaltered for the XP cells. Alkaline sucrose gradient centrifugation of DNA after UV irradiation revealed only a slight difference between FL and BSC-1 cells in the kinetics of formation of single-strand breaks and their apparent repair. From these and the previously known characters of L cells possessing reduced excision-repair ability, if any, we may conclude that, if the survival of UV-irradiated Herpes simplex virus on a test line of human or other mammalian cells is as low as that on excisionless XP cells, then it is very probable that the test cell line is defective in excision repair. This reasoning leads to the presumptive conclusion that mouse L cells have an enhanced post-replication repair other than excision repair to deal with UV damage responsible for inactivation of colony-forming ability. |
| |
Keywords: | MEM minimal essential medium PBS phosphate-buffered saline SDS sodium dodecyl sulfate XP xeroderma pigmentosum |
本文献已被 ScienceDirect 等数据库收录! |
|