A tubular segmented-flow bioreactor for the infection of insect cells with recombinant baculovirus |
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Authors: | Yu-Chen Hu Ming-Ying Wang William E. Bentley |
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Affiliation: | (1) Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, USA;(2) Department of Chemical Engineering, University of Maryland, College Park, MD, 20742 |
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Abstract: | A continuous process of insect cell (S f9) growth and baculovirus infection is tested with the sequential combination of a CSTR and a tubular reactor. A tubular infection reactor enables continuous introduction of baculovirus and therefore avoids the ‘passage effect’ observed in two-stage CSTR systems. Moreover, a tubular reactor can be used to test cell infection kinetics and the subsequent metabolism of infected insect cells. Unlike batch and CSTR culture, cells in a horizontally positioned tubular reactor settle due to poor mixing. We have overcome this problem by alternately introducing air bubbles and media and by maintaining a linear velocity sufficient to keep cells suspended. This article addresses the development of the tubular reactor and demonstrates its use as an infection system that complements the two-stage CSTR. This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | tubular reactor baculovirus infection insect cell culture |
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