The Dentin Matrix Protein 1 Gene of Prototherian and Metatherian Mammals |
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Authors: | Satoru Toyosawa Colm O'hUigin Jan Klein |
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Institution: | (1) Max-Planck-Institut für Biologie, Abteilung Immungenetik, Corrensstrasse 42, D-72076 Tübingen, Germany, DE |
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Abstract: | Mineralization of tooth dentin (the deposition of hydroxyapatite crystals in and around collagen type I fibers of the extracellular
matrix) requires the involvement of several genes, among them the gene coding for the dentin matrix protein 1, DMP1. We determined the exon–intron organization of the cattle DMP1 gene and used this information to amplify by the polymerase chain reaction homologous gene fragments from the genomic DNA
of two species of metatherian (marsupial) mammals and one prototherian (monotreme) species. The translated proto- and metatherian
protein sequences are highly divergent from the eutherian sequences but retain the general characteristics of the DMP1 (high
acidity, serine-richness, multiple glycosylation sites, and the presence of the RGD cell attachment tripeptide). They therefore
appear to be functional even though, evolutionarily, teeth are in a regression phase in prototherians. It is possible, therefore,
that DMP1 is also involved in other functions besides dentinogenesis. The DMP1 gene appears to evolve rapidly and apparently tolerates non-frame-shifting insertions/deletions throughout the coding sequence.
Received: 22 February 1998 / Accepted: 11 July 1998 |
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Keywords: | : Dentin — Phosphoprotein — Dentin matrix protein 1 — Marsupials — Monotremes |
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