Characterisation of HFBII biosurfactant production and foam fractionation with and without antifoaming agents |
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Authors: | James B Winterburn Andrew B Russell Peter J Martin |
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Institution: | (1) School of Chemical Engineering and Analytical Science, The Mill, The University of Manchester, Manchester, M13 9PL, UK;(2) Unilever R&D, Colworth Science Park, Sharnbrook, Bedford, MK44 1LQ, UK; |
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Abstract: | The effects of foaming on the production of the hydrophobin protein HFBII by fermentation have been investigated at two different
scales. The foaming behaviour was characterised in standard terms of the product enrichment and recovery achieved. Additional
specific attention was given to the rate at which foam, product and biomass overflowed from the fermentation system in order
to assess the utility of foam fractionation for HFBII recovery. HFBII was expressed as an extracellular product during fed-batch
fermentations with a genetically modified strain of Saccharomyces cerevisiae, which were carried out with and without the antifoam Struktol J647. In the presence of antifoam, HFBII production is shown
to be largely unaffected by process scale, with similar yields of HFBII on dry matter obtained. More variation in HFBII yield
was observed between fermentations without antifoam. In fermentations without antifoam, a maximum HFBII enrichment in the
foam phase of 94.7 was measured with an overall enrichment, averaged over all overflowed material throughout the whole fermentation,
of 54.6 at a recovery of 98.1%, leaving a residual HFBII concentration of 5.3 mg L−1 in the fermenter. It is also shown that uncontrolled foaming resulted in reduced concentration of biomass in the fermenter
vessel, affecting total production. This study illustrates the potential of foam fractionation for efficient recovery of HFBII
through simultaneous high enrichment and recovery which are greater than those reported for similar systems. |
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