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Two isoforms of acid phosphatase secreted by tobacco protoplasts: differential effect of brefeldin A on their secretion |
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| Authors: | Takako S. Kaneko Mamiko Sato Masako Osumi Makoto Muroi Akira Takatsuki |
| Affiliation: | (1) Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Mejiro-dai, Bunkyo-ku, 112 Tokyo, Japan;(2) Laboratory of Electron Microscopy, Japan Women's University, Mejiro-dai, Bunkyo-ku, 112 Tokyo, Japan;(3) Animal and Cellular Systems Laboratory, The Institute of Phys'l and Chem'l Research (RIKEN), Hirosawa, Wako-city, 351-01 Saitama, Japan |
| Abstract: | Summary The effects of brefeldin A (BFA) on the secretion of acid phosphatase (APase) by tobacco protoplasts were investigated. Secretion of APase was inhibited by BFA in a dose-dependent manner, with a concomitant intracellular accumulation of the enzyme. The secreted APase was composed of two isoforms. BFA (10/  g/ml) inhibited the secretion of one of the isoforms without inhibiting that of the other, and this phenomenon explains the partial inhibition of APase secretion as a whole. The inhibition of APase secretion was accompanied by changes in the morphology of the Golgi apparatus and also by an increment in massdensity of cells.Abbreviations APase acid phosphatase - BFA brefeldin A - CHX cycloheximide - PAGE polyacrylamide gel electrophoresis |
| Keywords: | acid phosphatase brefeldin A Nicotiana tabacum L. var. Xanthi secretion tobacco cells tobacco protoplasts |
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