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Decontamination ofFusarium mycotoxins,nivalenol, deoxynivalenol,and zearalenone,in barley by the polishing process
Authors:U S Lee  M-Y Lee  W-Y Park  Y Ueno
Affiliation:1. Department of Food Technology, National Chung-ju Technical College, Jungwon-gun, Chung-buk, 383-870, Korea
2. Department of Pharmacognosy, College of Pharmacy, Chung-buk National University, Cheong-ju, 360-763, Chung-buk, Korea
3. Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Ichigaya, Shinjuku-ku, 162, Tokyo, Japan
Abstract:Korean dehusked and unhusked barley naturally contaminated withFusarium mycotoxins were polished using a Satake Grain Testing Mill. The pearled barley and bran fractions with different degrees of polishing were analyzed for nivalenol (NIV) and deoxynivalenol (DON) by gas chromatography with an electron capture detector, and for zearalenone (ZEN) by high-performance liquid chromatography with a fluorescence detector. NIV was detected in all the pearled barley fractions, but DON and ZEN were not detected in ≥27 % pearled barley fractions from dehusked barley and ≥36% pearled barley fractions from unhusked barley. However, for all degrees of polishing, NIV, DON, and ZEN were detected in bran fractions. The levels of NIV, DON, and ZEN in the bran fractions increased several fold over the original barley. Polishing was effective in removing DON and ZEN from the naturally contaminated barley, but not NIV.
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