Identification of peanut agglutinin-binding glycoproteins on immature human thymocytes |
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| Authors: | A De Maio H Lis J M Gershoni N Sharon |
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| Affiliation: | 1. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;2. University of Chinese Academy of Science, Beijing 100049, China;3. Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;4. Sino-Czech Joint Laboratory for Fish Conservation and Biotechnology, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;5. University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic;1. School of Korean Medicine and Korean Medicine Research Center for Healthy Aging, Pusan National University, Yangsan, 50612, Republic of Korea;2. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, 08826, Republic of Korea;3. Department of Clinical Pathology, TaeKyeung University, Gyeongsan, 38547, Republic of Korea;4. Department of Biological Science, Sungkyunkwan University, Suwon, Kyunggi-do 16419, Republic of Korea;3. Institute for Clinical Chemistry, University of Cologne, D-50924 Cologne, Germany;5. Center for Biochemistry, Medical Faculty, University of Cologne, D-50931 Cologne, Germany;4. Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center (BMC), Lund University, SE-221 00 Lund, Sweden;6. Institute for Dental Research and Oral Musculoskeletal Biology, Medical Faculty, University of Cologne, D-50931 Cologne, Germany;12. Center for Molecular Medicine Cologne (CMMC), University of Cologne, D-50931 Cologne, Germany;8. Cologne Excellence Cluster on Cellular Stress Responses in Ageing-associated Diseases (CECAD), University of Cologne, D-50931 Cologne, Germany |
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| Abstract: | Previous studies in our laboratory have shown that peanut agglutinin (PNA), a lectin specific for the disaccharide Gal beta 3GalNAc, binds to immature (cortical) thymocytes of mouse and man and not to the mature (medullary) cells. Using lectin overlay of protein blots and lectin-affinity chromatography, we have found that the major PNA-binding glycoproteins on total as well as on immature (PNA+) human thymocytes correspond to two bands of Mr 170,000 and 180,000. Another glycoprotein, of Mr 110,000, also binds PNA but to a lesser extent. All three glycoproteins contain sialic acid as demonstrated by cell surface labeling with NaIO4-NaB3H4, binding of wheat germ agglutinin, and reaction with alkaline phosphatase-hydrazide. After treatment with sialidase, binding of PNA to these glycoproteins is significantly enhanced. |
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