Enzyme-catalyzed synthesis of optically pure R(+)-phenylethanol |
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Authors: | Werner Hummel |
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Affiliation: | (1) Institut für Enzymtechnologie der Heinrich-Heine-Universität, Forschungszentrum Jülich (KFA), PO Box 2050, D-5170 Jülich |
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Abstract: | Summary A dehydrogenase catalyzing the NADPH-dependent reduction of acetophenone to R(+)-phenylethanol was found inLactobacillus kefir. A continuous conversion of 10 mM acetophenone with a yield of up to 90% was achieved in an enzyme-membrane-reactor with simultaneous NADPH-regeneration using glucose-6-phosphate and glucose-6-phosphate dehydrogenase. Enzyme-catalyzed oxidation of phenylethanol occurred with the R(+)-enantiomer only, whereas S(–) was not converted by the enzyme. The formation of R(+)-phenylethanol with an enantiomeric excess of 100% was confirmed by chiral HPLC. |
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