Correlations between ovarian follicular blood flow and superovulatory responses in ewes |
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| Institution: | 1. Department of Preventative Veterinary Medicine and Animal Reproduction, College of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo 14.884-900, Brazil;2. Department of Animal Reproduction and Veterinary Radiology, Faculty of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo 18.618-970, Brazil;3. Brazilian Agricultural Research Corporation-Embrapa Goats and Sheep, Coronel Pacheco, Minas Gerais 36.155-000, Brazil;4. Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada;1. Laboratorio de Reproducción Animal, Polo Agroalimentario y Agroindustrial-CENUR Noroeste, Departamento de Salud en los Sistemas Pecuarios, Facultad de Veterinaria, Universidad de la República,Paysandú, Uruguay;2. Instituto Nacional de Investigación Agropecuaria, Programa Nacional de Carne y Lana (INIA), Uruguay;3. Consejo de Educación Técnico Profesional (CETP), Escuela Agraria La Carolina, Flores, Uruguay;1. Faculty of Veterinary Medicine, Ceará State University (UECE), Fortaleza, CE, Brazil;2. Molecular and Developmental Biology Lab, Health Sciences Center, University of Fortaleza (UNIFOR), Fortaleza, CE, Brazil;3. School of Veterinary Medicine, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil;4. Pontificial Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil;1. Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt;2. Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Tokyo, Japan;3. Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt;4. Sheep & Goats Research Department, Animal Production Research Institute, Ministry of Agriculture, Giza, Egypt;1. Embrapa Goats and Sheep Research Center, Estrada Sobral/Groaíras, km 04, CP 145, CEP 62010-970, Sobral, CE, Brazil;2. Faculty of Veterinary Medicine, Fluminense Federal University, Av. Vital Brasil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil;3. Department of Preventative Veterinary Medicine and Animal Reproduction, School of Agricultural and Veterinary Sciences, São Paulo State University, Via de acesso Prof. Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SP, Brazil;4. Department of Animal Science, Viçosa Federal University, Av. P.H. Rolfs, s/n, CEP 36571-000, Viçosa, MG, Brazil;5. Capril Sanri, Av. Constelações 385/242, Vale dos Cristais, CEP 34000-000, Nova Lima, MG, Brazil;1. Department of Pathology and Veterinary Clinic, Veterinary School, Fluminense Federal University, Niteroi, RJ 24320-340, Brazil;2. Brazilian Agricultural Research Corporation on Goats and Sheep, Coronel Pacheco, MG 36155-000, Brazil;3. Department of Animal Science, Veterinary School, Fluminense Federal University, Niteroi, RJ 24320-340, Brazil;1. Department of Preventative Veterinary Medicine and Animal Reproduction, College of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo, Brazil;2. Department of Clinical and Veterinary Surgery, College of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo, Brazil;3. Department of Animal Reproduction and Veterinary Radiology, Faculty of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, Brazil;4. Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada;5. Brazilian Agricultural Research Corporation-Embrapa Goats and Sheep, Coronel Pacheco, Minas Gerais, Brazil |
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| Abstract: | The primary goal of this study was to employ ultrasonography to examine the ovaries of ewes undergoing superovulatory treatment for correlations between antral follicular blood flow and ovarian responses/embryo yields. Five Santa Inês ewes were subjected to a short- (Days 0–6, Group 1) and five to a long-term progesterone-based protocol (Days 0–12, Group 2) to synchronize estrus and ovulations after the superovulatory treatment. Porcine FSH (pFSH, 200 mg) was administered in 8 decreasing doses over 4 days, starting on Days 4 and 10 in Groups 1 and 2, respectively. After CIDR removal, all ewes were bred by a ram and embryos were recovered surgically 7 days later. Transrectal ovarian ultrasonography was performed the day before and on all 4 days of the superovulatory treatment. Both an arbitrary-scale (0) non-detectable; (1) small; (2) moderate; (3) intense blood flow] and quantitative analysis of the blood flow area were used to assess the follicular blood flow in color Doppler images. There were no significant correlations between the arbitrary blood flow scores and superovulatory responses in the ewes of the present study. However, there was a positive correlation between the quantitative estimates of follicular blood flow on the final day of the superovulatory treatment, and the number (DA: r = 0.68, P < 0.05; DA/TA × 100%: r = 0.85, P < 0.05) and percentage (DA: r = 0.65, P < 0.05; DA/TA × 100%: r = 0.91, P < 0.001) of unfertilized eggs (DA: Doppler area, TA: total area of the largest ovarian cross section). This experiment presents a commercially practical tool for predicting superovulatory outcomes in ewes and evidence for the existence of follicular blood flow threshold that may impinge negatively on oocyte quality when surpassed during hormonal ovarian superstimulation. |
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| Keywords: | Ewe Superovulation Color Doppler sonography Blood flow Antral follicles |
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