Influence of oocyte donor on in vitro embryo production in buffalo |
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| Affiliation: | 1. Faculty of Veterinary Medicine, Department of Reproduction, Obstetrics and Herd Health, Ghent University, Merelbeke, Belgium;2. Department for Reproductive Medicine, Ghent University Hospital, Ghent, Belgium;3. Animal Breeding and Genomics Centre, Wageningen UR Livestock Research, Wageningen, The Netherlands;1. Faculdade de Veterinária, Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil;2. Embrapa Caprinos e Ovinos, Estrada Sobral/Groaíras, CEP 62010-970, Sobral, CE, Brazil;3. Embrapa Caprinos e Ovinos, Núcleo Regional Sudeste, Rodovia MG 133 Km 42, CEP 36155-000, Coronel Pacheco, MG, Brazil;1. Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, 50 Stone Road, Guelph, ON N1G 2W1, Canada;2. Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Corrêa 1, Belém, PA 66075-110, Brazil;3. School of Computer Science, University of Guelph, 50 Stone Road, Guelph, ON N1G 2W1, Canada;4. ONE Fertility, 3210 Harvester Rd, Burlington, ON L7N 3T1, Canada;1. Department of Animal Reproduction, FMVZ-USP, Campus São Paulo, São Paulo, Brazil;2. Agrindus, Descalvado, São Paulo, Brazil;3. Bioembryo, Bauru, São Paulo, Brazil;4. Department of Animal Reproduction, UNESP, Jaboticabal Campus, São Paulo, Brazil;5. Sexing Technologies Company, Sertãozinho, São Paulo, Brazil;6. Station of the Sertãozinho Institute of Zootechny, (EEZS), Sertãozinho, São Paulo, Brazil;7. Genese Company, São Paulo, São Paulo, Brazil;1. Unidad de Tecnología en Producción Animal, Centro de Investigación y Tecnología Agroalimentaria (CITA) de Aragón, Zaragoza, Spain;2. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain;3. INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly, France;4. CNRS, UMR7247, Nouzilly, France;5. Université François Rabelais de Tours, Tours, France;6. IFCE, Nouzilly, France;1. Department of Preventive Veterinary Medicine and Animal Reproduction, FCAV-UNESP, Jaboticabal, Brazil;2. Vida Reprodutiva Consultoria, Cravinhos, Brazil;3. Department of Surgery, FMVZ-USP, Pirassununga, Brazil;4. Department of Genetics and Evolution, CCBS-UFSCar, São Carlos, Brazil;5. Department of Animal Nutrition and Production, FMVZ-USP, Pirassununga, Brazil;6. Department of Animal Reproduction, FMVZ-USP, São Paulo, Brazil;7. Department of Veterinary Medicine, FZEA-USP, Pirassununga, Brazil;8. Department of Biostatistics, IB-UNESP, Botucatu, Brazil;9. Vitrogen, Cravinhos, Brazil |
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| Abstract: | The aim of this research was to estimate the variability between buffalo as oocyte donors. In Experiment 1, reproductive variables were retrospectively analyzed in buffalo (n = 40) that underwent repeated ovum pick up (OPU), over 16 puncture sessions (PS). The follicular recruitment among individuals and the relationship between follicular population and oocyte production were evaluated. In Experiment 2, eight buffalo underwent OPU for 28 PS and the oocytes were processed separately to correlate follicular and oocyte population at the first PS to blastocyst (BL) production. In Experiment 1, the average number of total follicles (TFL), small follicles (SFL), cumulus-oocyte complexes (COC) and Grade A + B COC recorded in each 4-PS period had great repeatability (r = 0.52, 0.54, 0.60 and 0.57, respectively). The average number of Grade A + B COC recovered during the subsequent 15 PS was positively correlated with the first PS number of TFL (r = 0.60; P < 0.001), SFL (r = 0.68; P < 0.001), COC (r = 0.48; P < 0.01) and Grade A + B COC (r = 0.40; P < 0.05). In Experiment 2, a large variability among animals was observed in blastocyst yields. When animals were grouped according to the BL yield, the greatest BL yield group had a greater (P < 0.05) number of TFL (8.3 ± 0.9 compared with 5.6 ± 0.7) and SFL (7.3 ± 0.3 compared with 3.8 ± 0.7) at the first PS than the lesser BL yield group. The average number of BL produced over the subsequent sessions was correlated with the number of TFL (r = 0.80; P < 0.05) and COC (r = 0.76; P < 0.05) observed at the first PS. These results demonstrated a donor influence on the oocyte and BL production, suggesting a preliminary screening to select the donors with greater potential. |
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| Keywords: | Buffalo Ovum pick-up Blastocyst yield Repeatability |
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