Synthesis of double-layered rotavirus-like particles using internal ribosome entry site vector system in stably-transformed <Emphasis Type="Italic">Drosophila melanogaster</Emphasis> |
| |
Authors: | Jong Min Lee Ha Young Chung Kyung Il Kim Ki Hyun Yoo Jeon Hwang-Bo In Sik Chung Jong-Hwa Park |
| |
Institution: | (1) Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Korea;(2) Department of Orthopaedics, Dongguk University Ilsan Hospital, Goyang, 814-820, Korea; |
| |
Abstract: | We established a bicistronic expression system using an encephalomyocarditis virus (EMCV)-derived internal ribosomal entry
site (IRES) element to generate stably transformed Drosophila
melanogaster Schneider 2 (S2) cells expressing human rotavirus Wa capsid proteins, VP2 and VP6, for the synthesis of VP2/6 double-layered
virus-like particle (DVLP). The EMCV-derived IRES permitted bicistronic translation of recombinant VP6. Recombinant VP2 and
VP6 were detected in extracellular fractions of stably transformed S2 cells. A wheel-like DVLP (diam ~ 50–55 nm) with short
spikes was produced from the extracellular fraction of stably transformed S2 cells. A bicistronic expression system using
an EMCV-derived IRES element can thus be used to express two proteins of interest in stably transformed S2 cells. The bi-or
tri-cistronic expression of recombinant VP2/6/7 using stably transformed S2 cells can also be used to produce rotavirus VLPs. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|