Molecular cloning and characterization of a novel gene, EMILIN-5, and its possible involvement in skeletal development |
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Authors: | Doi Mitsuhito Nagano Akira Nakamura Yusuke |
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Affiliation: | Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. |
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Abstract: | By analyzing expression profiles of human mesenchymal stem cells incubated in osteogenic supplements, we identified and characterized a novel human cDNA, elastin microfibril interface located protein-5 (EMILIN-5), that is likely to play a significant role in the process of osteogenesis. The deduced EMILIN-5 product consists of 766 amino acids with a cysteine-rich EMI domain at the NH(2) terminus. Western blotting detected EMILIN-5 expression in a variety of osteoblastic cell lines. Immunohistochemistry of mouse embryos 13.5 days post-coitus revealed relatively high levels of EMILIN-5 protein in perichondrium cells of developing limbs. Our findings suggest that the EMILIN-5 gene plays an important role in skeletal development. |
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Keywords: | cDNA microarray Bone EMILIN Mesenchymal stem cells Mineralization Osteogenesis Perichondrium Skeletal development |
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