News & Notes: The Effect of Ethanol and Oxygen on the Growth of Zymomonas mobilis and the Levels of Hopanoids and Other Membrane Lipids |
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Authors: | Robert A Moreau Michael J Powell William F Fett Bruce D Whitaker |
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Institution: | (1) Eastern Regional Research Center, USDA, Agricultural Research Service, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA , US;(2) Horticultural Crop Quality Lab, USDA, Agricultural Research Service, BARC-West Beltsville, MD 20705, USA , US |
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Abstract: | Zymomonas mobilis (ATCC 29191) was grown either aerobically or anaerobically in the presence of 2% (wt/vol) glucose and 0, 3, or 6% (vol/vol)
ethanol. The rates of growth and the composition of hopanoids, cellular fatty acids, and other lipids in the bacterial membranes
were quantitatively analyzed. The bacterium grew in the presence of 3% and 6% ethanol and was more ethanol tolerant when grown
anaerobically. In the absence of ethanol, hopanoids comprised about 30% (by mass) of the total cellular lipids. Addition of
ethanol to the media caused complex changes in the levels of hopanoids and other lipids. However, there was not a significant
increase in any of the hopanoid lipid classes as ethanol concentration was increased. As previously reported, vaccenic acid
was the most abundant fatty acid in the lipids of Z. mobilis, and its high constitutive levels were unaffected by the variations in ethanol and oxygen concentrations. A cyclopropane
fatty acid accounted for 2.6–6.4 wt % of the total fatty acids in all treatments.
Received: 12 November 1996 / Accepted: 25 February 1997 |
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