Solubilization and characterization of [3H]Imipramine and [3H]Paroxetine binding sites from calf striatum |
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| Authors: | A Rotondo G Giannaccini C Quattrone D Marazziti C Martin G B Cassano A Lucacchini |
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| Institution: | (1) Institute of Psychiatry, University of Pisa, via Roma 67, 56100 Pisa, Italy;(2) Instituto Policattedra di Discipline Biologiche, University of Piva, via Roma 67, 56100 Pisa, Italy |
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| Abstract: | The serotonin (5-HT) transporter from calf striatum cerebral membranes was solubilized with digitonin and characterized by gel exclusion chromatography. 3H]Imipramine and 3H]paroxetine were utilized as markers for labeling it.3H-imipramine labels a high- and a low-affinity site on striaturn membranes, whereas it binds to a single high-affinity site on the solubilized fraction. 3H]Paroxetine binds with the same affinity to a single site on both membranes and solubilized preparations. After gel exclusion chromatography of the solubilizate both 3H]imipramine and 3H]paroxetine bind on an identical fraction of 205 kDa molecular weight, with a similar maximum number of binding sites (Bmax). Our results suggest that both3H-imipramine and 3H]paroxetine bind to a common site on the 5-HT transporter. |
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| Keywords: | Serotonin neuronal serotonin transporter calf striatum [3H]imipramine binding [3H]paroxetine binding purification |
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