Cold-sensitive chromosome regions and heterochromatin inCestrum aurantiacum (Solanaceae) |
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Authors: | Christine Berg Johann Greilhuber |
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Affiliation: | (1) Institute of Botany, University of Vienna, Rennweg 14, A-1030 Wien, Austria |
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Abstract: | The karyotype ofCestrum aurantiacum was analyzed for the presence of coldsensitive regions (CSRs) and other types of constitutive heterochromatin. A range of techniques was employed including the fluorescent DAPI, chromomycin/DAPI double staining and actinomycin D/DAPI counter-staining, and the non-fluorescent C-banding applied as single or sequential staining, sequential N-banding and silver impregnation. Four classes of constitutive heterochromatin were recognized: CSRs, nucleolar organizers, non-nucleolar chromomycin-positive bands, and indifferently fluorescent bands. The banded karyotype ofC. aurantiacum is compared with those of otherCestrum species. The sectionsHabrothamnus andCestrum are not karyologically distinct. |
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Keywords: | Solanaceae Cestrum aurantiacum Cold-sensitive regions heterochromatin chromosome banding silver impregnation |
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