Crystallization and preliminary X-ray crystallographic analysis of human Geminin coiled-coil domain |
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Authors: | Thépaut Michael Hoh François Dumas Christian Calas Bernard Strub Marie-Paule Padilla André |
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Affiliation: | Centre de Biochimie Structurale, CNRS UMR 5048 INSERM UMR 554, Faculté de Pharmacie, 15 Av Charles Flahault, 34060 Montpellier, France. |
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Abstract: | Initially discovered in Xenopus laevis, Geminin is a DNA replication initiation inhibitor found in higher eukaryotes. The coiled-coil domain of Human Geminin (termed GemH-37) has been crystallized by the vapor-diffusion sitting-drop method. A complete 1.74 A data set has been collected on a single orthorhombic crystal with unit cell parameters a = 25.25, b = 44.35, c = 68.58 A. Successful molecular replacement shows that GemH-37 is a dimeric parallel coiled-coil. Structural analysis is now in progress. |
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