LaminB1蛋白在食管鳞癌组织中表达的形态学观察

目的探讨laminB1蛋白在食管鳞癌患者的癌组织及上切缘正常粘膜上皮中表达的形态变化。方法制备组织切片原位核基质,应用免疫组化的方法检测核基质制备前后正常粘膜上皮及癌组织中laminB1的表达;同时提取组织核基质蛋白,应用Westen blot检测核基质蛋白中laminB1的表达。结果正常食管粘膜上皮及食管鳞癌组织核基质制备前后laiminB1表达的阳性率分别为:正常粘膜上皮93.3%、正常粘膜上皮核基质86.7%、癌组织96.7%、癌核基质86.7%。正常粘膜上皮laminB1表达阳性细胞从基底层至颗粒层逐渐减少,制备核基质后正常粘膜上皮核基质laminB1表达阳性细胞数目减少、强度明显减弱,阳性细胞集中于基底层,多数细胞整个胞核着色。癌组织laminB1表达阳性细胞散在分布,无规律;癌核基质laminB1表达阳性强度减弱,阳性颗粒在核周较集中。癌核基质laminB1表达的阳性强度比正常粘膜上皮核基质高,差异有显著性(x2=5.042,P<0.05)。Western blot检测显示正常粘膜核基质的laminB1条带比癌核基质弱。结论laminB1蛋白在食管正常粘膜上皮及食管鳞癌组织中广泛存在。制备核基质后,laminB1蛋白在癌核基质的表达比正常粘膜上皮核基质强;且癌核基质中laminB1的分布与正常粘膜上皮核基质存在差异。

THE OBSERVATION OF LAMINB1 EXPRESSION IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA

Objective To investigate the change of laminB1 expression in normal esophageal epithelia and esophageal squamous cell carcinoma(ESCC).Methods We prepared frozen section nuclear matrix in situ,detected the expression of laminB1 proteins before and after preparing nuclear matrix in normal epithelia and ESCC tissues using immunohistochemistry.We prepared nuclear matrix protein and detected the expression of laminB1 proteins in normal mucosa and ESCC using Western blot.Results The positive rate of normal epithelia,nuclear matrix of normal epithelia,carcinoma,and nuclear matrix of carcinoma were 93.3%,86.7%,96.7% and 86.7% respectively.In normal epithelia,the expression level of laminB1 diminished from basal cells to granular cells.In nuclear matrix of normal epithelia,the expression level decreased and most positive cells were basal cells.Most positive cells showed whole nuclear staining.In ESCC,laminB1 expression showed no regular staining patterns.In nuclear matrix of carcinoma tissue,the expression level of laminB1 was reduced and showed a rim in the nuclear periphery.The expression level in nuclear matrix of carcinoma was higher than in nuclear matrix of normal epithelia,and the difference between them was significant(x2=5.042,P<0.05).Western blot analysis showed that the bands of laminB1 in normal mucosa nuclear matrix protein were weaker staining than in carcinoma nuclear matrix protein.Conclusion LaminB1 proteins are expressed in most normal esophageal epithelia and esophageal squamous cell carcinoma.The expression level of laminB1 protein in nuclear matrix of carcinama is higher than in the nuclear matrix of normal epithelia.The distribution of laminB1 protein in nuclear matrix of carcinoma was different from that in the nuclear matrix of normal epithelia.