Gene transfer in primary cultures of human hepatocytes |
| |
| Authors: | A. P. LI C. A. Myers D. L. Kaminski |
| |
| Affiliation: | (1) Monsanto Company, S2F, 800 N. Lindbergh Blvd., 63167 St. Louis, MO;(2) Health Sciences Department, Monsanto Corporate Research, Monsanto Company (A. P. L., C. A. M.), USA;(3) Department of Surgery, St. Louis of Medicine (A. P. L., D. L. K.), 63167 St. Louis, Missouri |
| |
| Abstract: | Summary Using liposomes as the mediator of DNA transfer, we were successful in the transfection of human hepatocytes isolated from surgical samples with an E. coli β-galactosidase gene (β-gal). A comparison of transfection efficiency showed that of the four promoters used, cytomegalovirus (CMV) promoter yielded higher transfection efficiencies than Rous sarcoma virus (RSV), Simian virus-40 (SV-40) and human alpha-l antitrypsin (AAT) promoters. These studies represent the first report on the successful transfection of primary cultures of human hepatocytes. |
| |
| Keywords: | human hepatocytes viral promoters liposome mediated DNA transfer transfection lipofection gene therapy. |
| 本文献已被 SpringerLink 等数据库收录！ |
|
