Full-term development of in vitro-matured, vitrified and fertilized bovine oocytes |
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Authors: | Hamano S Koikeda A Kuwayama M Nagai T |
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Affiliation: | Tokyo Bio-Technology Center, Livestock Improvement Association Inc. 3-21-10 Higashishinagawa, Shinagawa-ku Tokyo 140, Japan. |
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Abstract: | In vitro-matured bovine oocytes were vitrified in a mixture of 2 M-dimethyl sulphoxide (DMSO), 1 M-acetamide and 3 M-propylene glycol dissolved in mTCM199. After vitrification and thawing, the oocytes were exposed to 2-0.1M-sucrose solution in 1 or 12 steps to remove the cryoprotectants. Then the oocytes were fertilized in vitro and co-cultured with a monolayer of cumulus cells for 7 days. Nine of 88 inseminated oocytes developed to the blastocyst stage. Three blastocysts were transferred to 3 recipients, resulting in 2 pregnancies. |
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