Immunohistochemical demonstration of renin in the juxtaglomerular apparatus of three Bufo species |
| |
| Authors: | Antoine P M Lamers Albert A J Verhofstad Ad M Stadhouders Andrew M Michelakis |
| |
| Institution: | (1) Department of Submicroscopic Morphology, University of Nijmegen, Medical School, The Netherlands;(2) Department of Anatomy and Embryology, University of Nijmegen, Medical School, The Netherlands;(3) Departments of Pharmacology and Toxicology, Michigan State University, East Lansing Michigan, USA;(4) Department of Submicroscopic Morphology, University of Nijmegen, Medical School, Geert Grooteplein Zuid 24, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands |
| |
| Abstract: | Summary The cellular localization of renin was examined in the kidneys of some amphibians of the genus Bufo by immunoperoxidase and immunofluorescence techniques with an antiserum to renin isolated from the submandibular gland of the mouse. Immunoreactivity could be demonstrated in the media cells of the afferent arterioles (juxtaglomerular cells) close to as well as at great distance from the glomeruli. Occasionally, media cells of larger arterial vessels were also stained. The immunohistochemical data seem to be in accordance with earlier results obtained with a modified silver impregnation technique (Movat's staining procedure) used for the visualization of juxtaglomerular cells in non-mammalian vertebrates. Mouse kidney tissue, studied for purposes of comparison, showed renin-immunoreactivity as described by earlier investigators, i.e., immunoreactive staining in the afferent arterioles near the glomeruli and in the proximal tubule cells. |
| |
| Keywords: | Juxtaglomerular cells Renin Immunohistochemistry Movat silver impregnation Bufo |
| 本文献已被 SpringerLink 等数据库收录！ |
|
