| 水稻LRR型类受体蛋白激酶胞外区的原核表达及多克隆抗体制备 |
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| 引用本文： | 程彦伟,李 亮,沈 嵘,齐耀程,刘晓宇,王 宁,张 炜. 水稻LRR型类受体蛋白激酶胞外区的原核表达及多克隆抗体制备[J]. 生物化学与生物物理进展, 2008, 35(9): 1077-1083 |
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| 作者姓名： | 程彦伟 李 亮 沈 嵘 齐耀程 刘晓宇 王 宁 张 炜 |
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| 作者单位： | 南京农业大学生命科学学院生物化学与分子生物学系南京农业大学生命科学学院生物化学与分子生物学系,南京农业大学GE Healthcare Biosciences蛋白质组学合作示范实验室,南京 210095 |
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| 基金项目： | 国家自然科学基金，教育部跨世纪优秀人才培养计划 |
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| 摘 要： | 前期研究表明,水稻根尖细胞质膜类受体蛋白激酶OsRLK的表达受盐胁迫诱导.为了进一步研究该激酶的生理功能,通过反转录PCR得到OsRLK胞外区cDNA片段,将其亚克隆至pET29a原核表达载体并在大肠杆菌中实现了高表达,表达量约为细胞总蛋白的30%.重组蛋白经SDS-PAGE分离,染色切胶收集后,作为抗原免疫新西兰家兔,分离抗血清,经纯化得到1:20 000效价的多克隆抗体.Wescem blot结果显示,该抗体能特异识别在原核表达系统内表达的抗原,以及水稻根尖细胞质膜组分中的LRR型类受体蛋白激酶,并且在蛋白质水平证实该激酶为盐胁迫响应蛋白.
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| 关 键 词： | LRR型类受体蛋白激酶 水稻 原核表达 多克隆抗体 |
| 收稿时间： | 2008-01-21 |
| 修稿时间： | 2008-04-06 |
Prokaryotic Expression and Polyclonal Antibody Preparation of The Extracellular Domain About Rice LRR Receptor-like Protein Kinase |
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| CHENG Yan-Wei,LI Liang,SHEN Rong,QI Yao-Cheng,LIU Xiao-Yu,WANG Ning and ZHANG Wei. Prokaryotic Expression and Polyclonal Antibody Preparation of The Extracellular Domain About Rice LRR Receptor-like Protein Kinase[J]. Progress In Biochemistry and Biophysics, 2008, 35(9): 1077-1083 |
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| Authors: | CHENG Yan-Wei LI Liang SHEN Rong QI Yao-Cheng LIU Xiao-Yu WANG Ning ZHANG Wei |
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| Affiliation: | College of Life Science, Nanjing Agricultural University, Nanjing Agricultural University GE Healthcare Biosciences Proteomics Research Cooperative Demonstration Laboratory, Nanjing 210095, China;College of Life Science, Nanjing Agricultural University, Nanjing Agricultural University GE Healthcare Biosciences Proteomics Research Cooperative Demonstration Laboratory, Nanjing 210095, China;College of Life Science, Nanjing Agricultural University, Nanjing Agricultural University GE Healthcare Biosciences Proteomics Research Cooperative Demonstration Laboratory, Nanjing 210095, China;College of Life Science, Nanjing Agricultural University, Nanjing Agricultural University GE Healthcare Biosciences Proteomics Research Cooperative Demonstration Laboratory, Nanjing 210095, China;College of Life Science, Nanjing Agricultural University, Nanjing Agricultural University GE Healthcare Biosciences Proteomics Research Cooperative Demonstration Laboratory, Nanjing 210095, China;College of Life Science, Nanjing Agricultural University, Nanjing Agricultural University GE Healthcare Biosciences Proteomics Research Cooperative Demonstration Laboratory, Nanjing 210095, China;College of Life Science, Nanjing Agricultural University, Nanjing Agricultural University GE Healthcare Biosciences Proteomics Research Cooperative Demonstration Laboratory, Nanjing 210095, China |
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| Abstract: | |
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| Keywords: | LRR receptor-like protein kinase Oryza sativa prokaryotic expression polyclonal antibody |
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