Molecular aspects in elevation of sunflower tolerance to drought by boron and calcium foliar sprays |
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Authors: | Nemat M Hassan Ahmed K A El-Sayed Heba T Ebeid Mamdouh M Nemat Alla |
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Institution: | (1) Botany Department, Faculty of Science at Damietta, Mansoura University, Damietta, Egypt; |
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Abstract: | Sunflower (Helianthus annuus L.) hybrid Hysun 333 (black seeded) was cultivated in a greenhouse and subjected during flowering stage to drought with or
without the application of boron (B) and calcium (Ca) foliar sprays alone or in combination. The results revealed that drought
induced a sharp decrease in seed fresh weight, seed protein and lipid contents. Application of B and Ca combined sprays overcame
the drought effects on seed weight and seed lipid contents. Catalase expression was detected as a single band for all treatments
where peroxidase isozymes were increased to seven, six of them were expressed when B sprays were applied with drought indicating
that B has a major role in peroxidase up-regulation under drought conditions. SDS-PAGE analysis showed differential changes
in protein profile with an appearance and/or disappearance of polypeptide protein bands, some of which were concluded to be
drought-related proteins. The combined sprays of B and Ca seemed to overcome the effects of drought through minimizing band
alterations (disappearance and/or appearance). The DD-RT PCR showed a variation in gene expression between the control and
the other treatments. Sprays of B and Ca in combination seemed to be the most effective in band up-regulation and/or down-regulation
that might play a possible role in improving tolerance of sunflower to overcome the drought deleterious effects. |
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