| 气升式液体培养假褐云斑鹅膏菌丝及毒素检测分析 |
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| 引用本文： | 汪国轮 郭学武 龚建华. 气升式液体培养假褐云斑鹅膏菌丝及毒素检测分析[J]. 菌物学报, 2005, 24(4): 543-550 |
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| 作者姓名： | 汪国轮 郭学武 龚建华 |
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| 作者单位： | 1. 中国科学院微生物研究所,北京,100080;中国科学院研究生院,北京,100039
2. 中国科学院微生物研究所,北京,100080 |
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| 基金项目： | 致谢：感谢中国科学院微生物研究所卯晓岚先生在鹅膏菌分类学方面的指导;感谢湖南师范大学张志光先生赠送致命鹅膏A.exitialis菌种;感谢陈作红教授、张平博士在蕈菌采集、菌种鉴定以及HPLC分析中给予的帮助. |
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| 摘 要： | 采集并组织分离到假褐云斑鹅膏Amanita pseudoporphyria的纯培养菌丝。培养发现其在琼脂培养基上生长形态与一般蕈菌有差别。摇瓶液体培养条件下,假褐云斑鹅膏菌丝体干重为0.626g/L,在气升式反应器内假褐云斑鹅膏菌丝体干重可达到1.629g/L。反应器培养的菌丝体毒素的HPLC分析表明菌丝体内含有鹅膏毒肽,而不含有鬼笔毒肽。结果表明可以通过液体培养鹅膏菌丝体来生产鹅膏毒素。
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| 关 键 词： | 鹅膏菌 纯培养菌丝 外生菌根真菌 HPLC分析 |
| 文章编号： | 1672-6472（2005）04-0543-0550 |
| 收稿时间： | 2005-01-07 |
| 修稿时间： | 2005-06-17 |
LIQUID CULTURE OF AMANITA PSEUDOPORPHYRIA IN AIRLIFT/FF BIOREACTOR AND TOXIN ANALYSIS OF MYCELIA |
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| WANG Guo-Lun,GUO Xue-Wu,GONG Jian-Hua. LIQUID CULTURE OF AMANITA PSEUDOPORPHYRIA IN AIRLIFT/FF BIOREACTOR AND TOXIN ANALYSIS OF MYCELIA[J]. Mycosystema, 2005, 24(4): 543-550 |
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| Authors: | WANG Guo-Lun GUO Xue-Wu GONG Jian-Hua |
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| Affiliation: | 1Institute of Microbiology, Chinese Academy of Sciences, 100080;2 Graduate School of Chmese Academy of Sciences, 100039 |
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| Abstract: | Sporocarps of Amanita pseudoporphyria was collected and the pure culture was obtained by way of tissue culture. It was found that colonial morphology of the fungus in Petri dish was different from that of most mushroom species. Dry mycelial weight of A. pseudoporphyria cultured in shaken flask reached 0.626mg/mL and it reached 1.629 g/L when cultured in Airlift/ff bioreactor. Toxin compositions of mycelia obtained from Airlift/ff bioreactor was analyzed by HPLC and it proved that mycelia contained amatoxins but no phallotoxins. The research result demonstrated that it was possible to produce amatoxins by way of liquid culture of A. pseudoporphyria. |
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| Keywords: | Amanita pure culture ectomycorrhizae HPLC analysis |
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