ATP-induced activation of expressed RyR3 at low free calcium |
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Authors: | Manunta M Rossi D Simeoni I Butelli E Romanin C Sorrentino V Schindler H |
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Institution: | Institute for Biophysics, University of Linz, Altenberger Strasse 69, A-4040, Linz, Austria. |
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Abstract: | Ca(2+) channel properties of the mink ryanodine receptor type 3 (RyR3), expressed in HEK293 cells, were studied in planar lipid bilayers to which RyR3 rich membrane fragments had been fused. RyR3 channels were not active at resting levels of Ca(2+)(free) but were gated by an additional 1 mM ATP, exhibiting long open times. The second major finding was the absence of channel inactivation at millimolar Ca(2+)(free). Insertion of a myc tag at the N-terminus of RyR3 did not affect the channel properties. As to skeletal muscle, the observed type 3 channel properties appear physiologically meaningful by assisting type 1 channels in calcium release. |
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