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Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method |
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| Authors: | Eriko Kage-Nakadai Hiroyuki Kobuna Osamu Funatsu Muneyoshi Otori Keiko Gengyo-Ando Sawako Yoshina Sayaka Hori and Shohei Mitani |
| Institution: | (1) Department of Physiology, Tokyo Women’s Medical University School of Medicine, Tokyo, Japan;(2) Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama, Japan;(3) Saitama University Brain Science Institute, Saitama, Japan |
| Abstract: | BackgroundTransgenic strains of Caenorhabditis elegans are typically generated by injecting DNA into the germline to form multi-copy extrachromosomal arrays. These transgenes are semi-stable and their expression is silenced in the germline. Mos1 transposon or microparticle bombardment methods have been developed to create single- or low-copy chromosomal integrated lines. Here we report an alternative method using ultraviolet trimethylpsoralen (UV/TMP) to generate single/low-copy gene integrations. |
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