Combining SNP discovery from next-generation sequencing data with bulked segregant analysis (BSA) to fine-map genes in polyploid wheat |
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| Authors: | Martin Trick Nikolai Maria Adamski Sarah G Mugford Cong-Cong Jiang Melanie Febrer Cristobal Uauy |
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| Institution: | (1) John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK;(2) The Genome Analysis Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK;(3) National Institute of Agricultural Botany, Huntingdon Road, Cambridge, CB3 0LE, UK |
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| Abstract: | Background Next generation sequencing (NGS) technologies are providing new ways to accelerate fine-mapping and gene isolation in many
species. To date, the majority of these efforts have focused on diploid organisms with readily available whole genome sequence
information. In this study, as a proof of concept, we tested the use of NGS for SNP discovery in tetraploid wheat lines differing
for the previously cloned grain protein content (GPC) gene GPC-B1. Bulked segregant analysis (BSA) was used to define a subset of putative SNPs within the candidate gene region, which were
then used to fine-map GPC-B1. |
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