Effects of chloramphenicol on brain energy metabolism using 31P spectroscopy: influences on sleep-wake states in rat |
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Authors: | Chahboune Halima Mahdjoub Rachid Desgoutte Pierre Rousset Colette Briguet André Cespuglio Raymond |
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Institution: | Anlyan Center for Medical Research, Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, Connecticut, USA; CNRS-UMR 5012, Claude Bernard University, Villeurbanne, France; EA 4170 &IFR19, Claude Bernard University, Lyon, France |
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Abstract: | Effects of chloramphenicol (antibiotic inhibiting complex-1 of respiratory chain) and thioamphenicol (TAP, a structural analog of CAP inactive on complex-1) were examined on cerebral energy metabolites and sleep-wake cycle architecture in rat. In the first group, animals were chronically equipped with a cranial surface resonator and 31P spectroscopic measurements were performed using a 2 T magnetic resonance spectrometer (operating frequency 34.46 MHz). CAP administration (400 mg/kg, tail vein, light period) induced deficits in phosphocreatine (−30%, p < 0.01) and ATP (−40%, p < 0.01), whereas TAP (400 mg/kg) had no effect. In the second group, animals were chronically implanted with polygraphic electrodes for EEG and electromyogram recordings. CAP administered intraperitoneally at light-onset reduced rapid-eye movement (REM) sleep (−60% in the first 6 h of light period, p < 0.01), increased waking state (+65% in the first 6 h of light period, p < 0.01), and slightly affected slow-wave sleep (SWS). During waking state, θ and σ power bands of the EEG were, respectively, increased and decreased ( p < 0.05). During SWS, delta power band was reinforced ( p < 0.05), while θ, α, and σ bands were decreased ( p < 0.05). No changes occurred during REM sleep. TAP had no effect on sleep-wake states and spectral components of the EEG. Overall, these data indicate that REM sleep occurrence is linked to an aerobic production of ATP. |
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Keywords: | adenosine triphosphate chloramphenicol rapid-eye-movements sleep |
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