首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Production and purification of a novel extracellular lipase from Alternaria brassicicola
Authors:Philippe Berto  Lionel Belingheri  Bertrand Dehorter
Institution:(1) Laboratoire de Cryptogamie-Phytopathologie, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France
Abstract:Alternaria brassicicola produced higher quantities (3.2 U/ml) of an inducible extracellular lipase (EC 3.1.1.3) in shaken synthetic medium supplemented with 20 mM methyloleate. After purification, the M r of the lipase was determined as 80 kDa by SDS-PAGE and estimated at 85 kDa using gel filtration, which suggest that the enzyme may be a monomer. The optimum pH and temperature for activity of the enzyme were 9.0 and 25ºC, respectively. Using umbelliferone esters, the lipase was shown highly specific towards a synthetic substrate with long-chain unsaturated fatty acid.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号