Effects of colchicine and cytochalasin B on hypertonicity-induced changes in toad urinary bladder |
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Authors: | Max Dratwa Ann LeFurgey C. Craig Tisher M.D. |
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Affiliation: | (1) Division of Nephrology, Department of Medicine, University of Florida, Gainesville, Florida, USA;(2) Departments of Medicine and Pathology, Duke University Medical Center, Durham, North Carolina, USA;(3) Division of Nephrology, JHMHC, University of Florida, Box J-224, 32610 Gainesville, Florida, USA;(4) Present address: Fondation Médicale Reine Elisabeth, Brussels, Belgium;(5) Present address: Department of Physiology, Duke University Medical Center, Durham, North Carolina, USA |
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Abstract: | Summary Coincident with an increase in the water permeability of toad urinary bladder induced by serosal hypertonicity, a transformation of the ridge-like surface structures of the granular cells into individual microvillous structures occurs. This study was initiated to establish whether the transformation is mediated by the cytoskeletal network and, thus, can be prevented by disruption of microtubulemicrofilament function with colchicine or cytochalasin B (CB). Scanning electron microscopy revealed the characteristic branching ridges on granular cells of control bladder incubated with colchicine or CB. In contrast, transformation of ridges to discrete microvilli was observed in experimental bladders exposed to serosal hypertonicity alone or in combination with either colchicine or CB. These results suggest that the mechanism underlying hypertonicity-induced surface changes which are associated with increased water permeability does not involve either microtubules or microfilaments. |
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Keywords: | Urinary bladder, toad Scanning electron microscopy Hypertonicity |
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