Inhibition of elongation factor 1-dependent aminoacyl-tRNA binding to ribosomes by Shiga-like toxin I (VT1) from Escherichia coli O157:H7 and by Shiga toxin |
| |
| Authors: | Kazuei Igarashi Toshihiro Ogasawara Kiyoshi Ito Takashi Yutsudo Yoshifumi Takeda |
| |
| Institution: | Department of Biology, New Mexico State University, Las Cruces, NM, U.S.A. |
| |
| Abstract: | A Vero toxin (VT1 or Shiga-like toxin I) from Escherichia coli O157:H7 was found to inactivate 60S ribosomal subunits of rabbit reticulocyte resulting in the inhibition of protein synthesis. The mode of inhibition of both VT1 and Shiga toxin was similar and involved the blocking of elongation-1-dependent binding of aminoacyl-tRNA of ribosomes. Non-enzymatic binding of aminoacyl-tRNA to ribosomes, peptide bond formation and translocation were not inhibited by either VT1 or Shiga toxin. |
| |
| Keywords: | Mucor rouxii Mucoric acid endo-β(1 4)-d-Glucuronidase Lysis |
| 本文献已被 ScienceDirect 等数据库收录！ |
