Inhibition of PTEN expression and activity by angiotensin II induces proliferation and migration of vascular smooth muscle cells |
| |
Authors: | Xue Dong Lu‐Gang Yu Rong Sun Yan‐Na Cheng Hua Cao Kang‐Min Yang Yi‐Ning Dong Yan Wu Xiu‐Li Guo |
| |
Institution: | 1. Department of Pharmacology, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, P.R. China;2. The Henry Wellcome Laboratory of Molecular and Cellular Gastroenterology, Liverpool Centre for Glycobiology, School of Clinical Sciences, University of Liverpool, Liverpool L69 3BX, UK;3. Department of Pharmacology, Shandong Academy of Traditional Chinese Medicine, Jinan 250014, P.R. China |
| |
Abstract: | PTEN (phosphatase and tensin homolog deleted on chromosome 10) is a tumor suppressor and has been suggested recently to be involved in the regulation of cardiovascular diseases. The molecular mechanisms of this regulation are however poorly understood. This study shows that down regulation of PTEN expression and activity by angiotensin II (Ang II) increased proliferation and migration of vascular smooth muscle cells (VSMCs). The presence of Ang II induced rapid PTEN phosphorylation and oxidation in accordance with increased AKT and FAK phosphorylation. The Ang II‐mediated VSMC proliferation and migration was inhibited when cellular PTEN expression was increased by AT1 inhibitor losartan, PPARγ agonist rosiglitazone, NF‐κB inhibitor BAY 11‐7082. Over expression of PTEN in VSMCs by adenovirus transduction also resulted in inhibition of cell proliferation and migration in response to Ang II. These results suggest that PTEN down‐regulation is involved in proliferation and migration of VSMCs induced by Ang II. This provides insight into the molecular regulation of PTEN in vascular smooth muscle cells and suggests that targeting the action of PTEN may represent an effective therapeutic approach for the treatment of cardiovascular diseases. J. Cell. Biochem. 114: 174–182, 2012. © 2012 Wiley Periodicals, Inc. |
| |
Keywords: | PTEN ANGIOTENSIN II VASCULAR SMOOTH MUSCLE CELLS PROLIFERATION MIGRATION |
|
|