Co‐expression of CD133+/CD44+ in human colon cancer and liver metastasis |
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Authors: | Antonia Bellizzi Sinto Sebastian Pasquale Ceglia Matteo Centonze Rosa Divella Elvira Foglia Manzillo Amalia Azzariti Nicola Silvestris Severino Montemurro Cosimo Caliandro Raffaele De Luca Giuseppe Cicero Sergio Rizzo Antonio Russo Michele Quaranta Giovanni Simone Angelo Paradiso |
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Institution: | 1. Invasion and Metastatization Laboratory, Department of Experimental Oncology, National Cancer Centre “Giovanni Paolo II”, Bari, Italy;2. Clinical Pathology Laboratory, Department of Experimental National Cancer Centre “Giovanni Paolo II”, Bari, Italy;3. Department of Pathology, National Cancer Centre “Giovanni Paolo II”, Bari, Italy;4. Clinical and Preclinical Pharmacology Unit, Department of Experimental National Cancer Centre “Giovanni Paolo II”, Bari, Italy;5. Medical and Experimental Oncology Unit, National Cancer Centre “Giovanni Paolo II”, Bari, Italy;6. Department of Surgical Oncology, National Cancer Centre “Giovanni Paolo II”, Bari, Italy;7. Section of Medical Oncology, Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy |
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Abstract: | Although relatively good therapeutic results are achieved in non‐advanced cancer, the prognosis of the advanced colon cancer still remains poor, dependent on local or distant recurrence of the disease. One of the factors responsible for recurrence is supposed to be cancer stem cells (CSCs) or tumor‐initiating cells, which are a population of cancer cells with ability to perpetuate themselves through self‐renewal and to generate differentiated cells, thought to be responsible for tumor recurrence. This study globally approach the possible role of tissue‐derived stem cells in the initiation of colon cancer and its metastatic process in the liver. Fresh surgical specimens from colon cancer, non‐tumor tissue and liver metastasis were obtained directly from the operating room, examined, and immediately processed. CSCs were selected under serum‐free conditions and characterized by CD44 and CD133 expression levels. CD133+/CD44+ cell populations were then investigated in paraffin‐embedded tissues and circulating tumor cells isolated from peripheral blood of the same group of colon cancer patients. Our data demonstrate that metastatic properties of cell populations from blood and liver metastasis, differently from primitive tumors, seem to be strictly related to the phenotype CD133 positive and CD44 positive. J. Cell. Physiol. 228: 408–415, 2013. © 2012 Wiley Periodicals, Inc. |
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