Growth of v‐src‐transformed cells in serum‐free medium through the induction of growth factors |
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Authors: | Tiziana DeAngelis Andrew Quong Andrea Morrione Renato Baserga |
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Institution: | 1. Department of Cancer Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania;2. Department of Cancer Urology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania |
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Abstract: | The v‐src oncogene is one of only two oncogenes capable of transforming mouse embryo fibroblasts (MEFs) lacking the IGF‐IR gene (R‐cells). R‐/v‐src cells grow robustly in the absence of serum, suggesting the hypothesis that they may produce one or more growth factors that would sustain their ability to proliferate in serum‐free condition. Using proteomic approaches on serum‐free conditioned media derived from v‐src‐transformed cells, we have identified two growth promoting factors: ostepontin and proliferin. Subsequent experiments have indicated that osteopontin plays a prevalent role in promoting growth of v‐src‐transformed cells in serum‐deprived condition. J. Cell. Physiol. 228: 1482–1486, 2013. © 2012 Wiley Periodicals, Inc. |
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