Towards 3D modelling and imaging of infection scenarios at the single cell level using holographic optical tweezers and digital holographic microscopy |
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| Authors: | Björn Kemper Álvaro Barroso Mike Woerdemann Lena Dewenter Angelika Vollmer Robin Schubert Alexander Mellmann Gert von Bally Cornelia Denz |
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| Institution: | 1. Center for Biomedical Optics and Photonics, University of Muenster, Robert‐Koch‐Str. 45, 48149 Muenster, Germany;2. Institute of Applied Physics, University of Muenster, Corrensstra?e 2/4 Muenster, 48149 Muenster, Germany;3. Institute of Hygiene, University of Muenster, Robert‐Koch‐Str. 41, 48149 Muenster, Germany |
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| Abstract: | The analysis of dynamic interactions of microorganisms with a host cell is of utmost importance for understanding infection processes. We present a biophotonic holographic workstation that allows optical manipulation of bacteria by holographic optical tweezers and simultaneously monitoring of dynamic processes with quantitative multi‐focus phase imaging based on self‐interference digital holographic microscopy. Our results show that several bacterial cells, even with non‐spherical shape, can be aligned precisely on the surface of living host cells and localized reproducibly in three dimensions. In this way a new label‐free multipurpose device for modelling and quantitative analysis of infection scenarios at the single cell level is provided. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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| Keywords: | holographic optical tweezers digital holographic microscopy infection monitoring live cell imaging bacteria |
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